Journal: Nature Communications

Article Title: Metal-organic polyhedra maintain the self-renewal of embryonic stem cells

doi: 10.1038/s41467-025-63811-6

Figure Lengend Snippet: A The photo of MOP-1 aqueous solution (left). Monodisperse structure of MOP-1 in H 2 O (right). Color codes: V, green; O, red; C, gray; N, blue; Cl, bright blue. The large pink sphere represents the free space inside the molecular cage. For clarity, H atoms were omitted. Schematic (right panel) was created with Diamond software. B Experimental and simulated PXRD patterns of MOP-1. C TEM image of MOP-1 (left). Scale bar, 20 nm. The particle size of MOP-1 in H 2 O (right). Three experiments were repeated independently with similar results. D The schematic diagram showed that the SHP-2 mediated STAT3 inactivation. Schematic diagram was created with Microsoft Office PowerPoint. E , F An in-depth mechanism investigation of mESC pluripotency control by MOP-1. Binding model from a global view of a complex composed of SHP-2 and MOP-1 illustrated by electrostatic surface potential ( E ). Binding modes are illustrated by ribbon diagrams of a complex composed of SHP-2 and MOP-1 (the left panel), a complex composed of SHP-2 with MOP (the middle panel) and a complex composed of SHP-2 with ZrMOP (the right panel). The top panel is the global view of the catalytic PTP structure of SHP-2, the bottom panel is the focused view of binding modes illustrated by the Ribbon diagrams ( F ). G Binding kinetics of MOP-1 (top panel) and MOP-2 (bottom panel) with SHP-2 were measured by the SPR assay. H The ICP-MS analysis of the binding quantity between MOPs and SHP-2 (mean ± s.e.m, n = 6). I The inhibition efficiency of SHP-2, JAK2, JAK1, SHP-1, PTP1B, Cyt c , ACP and lipase by MOP-1 at a concentration of 2 μM using enzyme assay (mean ± s.e.m, n = 3). Data in ( H ) and ( I ) are analyzed by one-way ANOVA. **** P < 0.0001, the binding between MOP-1 and SHP-2 vs. the binding between MOP-2 and SHP-2, relative activity of SHP-2 vs. relative activity of JAK2, JAK1, PTP1B, Cyt c , ACP and lipase. *** P < 0.001, relative activity of SHP-2 vs. relative activity of SHP-1.

Article Snippet: The binding kinetics and affinity of MOP-1 or MOP-2 to SHP-2 (MCE, HY-P700618), SHP-1 (MCE, HY- P71141 ), JAK1 (MCE, HY-P700583), JAK2 (MCE, HY-P701102) and PTP1B (MCE, HY- P73685 ) were analyzed by SPR (Biacore 8 K, Cytiva).

Techniques: Software, Control, Binding Assay, SPR Assay, Inhibition, Concentration Assay, Enzymatic Assay, Activity Assay

Journal: Toxicology and applied pharmacology

Article Title: PTP1B confers liver fibrosis by regulating the activation of hepatic stellate cells.

doi: 10.1016/j.taap.2015.12.021

Figure Lengend Snippet: Fig. 2. Up-regulation of PTP1B in the fibrotic livers from CCl4-treated mice and down-regulation in the spontaneous recovery livers, n = 12 per group. a. The level of PTP1B and α-SMA were analyzed by immune-histochemistry in control, model and recovery livers. Representative views from each group are presented (×100). b. The expression of PTP1B, Col1α1 and α-SMA mRNA was assessed by Real-time PCR. The results are expressed as relative expression against negative control expression. The graph represents mRNA expression as mean ± SD from three independent experiments.⁎⁎p b 0.01 vs control; #p b 0.05, ##p b 0.01 vs model. c. The expression of PTP1B, Col1α1 and α-SMA protein was assessed by Western blot. The results are expressed as relative expression against negative control expression. Representative blots of three independent experiments are shown. ⁎⁎p b 0.01 vs control; #p b 0.05, ##p b 0.01 vs model. d. Double immunofluorescence staining was performed to determine the colocalization of PTP1B (green) and α-SMA (red) in CCl4-induced mouse liver fibrosis models. Representative views from each group are presented (×100).

Article Snippet: Rabbit polyclonal anti-aSMA (Proteintech, USA) was diluted at 1:500, Rabbit polyclonal anti -PTP1B and Col1α1 (Boster, China) were diluted 1:200; mouse monoclonal anti-β-actin was diluted 1:200.

Techniques: Control, Expressing, Real-time Polymerase Chain Reaction, Negative Control, Western Blot, Staining

Journal: Toxicology and applied pharmacology

Article Title: PTP1B confers liver fibrosis by regulating the activation of hepatic stellate cells.

doi: 10.1016/j.taap.2015.12.021

Figure Lengend Snippet: Fig. 3. Increased expression of PTP1B during HSC activation induced by TGF-β1. a. HSC-T6 cells were stimulated with different concentrations of TGF-β1 (0, 5, 10 ng/ml) for 24 h. The mRNA level of PTP1B, Col1a1 and α-SMA were examined by Real-time PCR; the protein level of PTP1B, Col1a1 and α-SMA were assessed by Western blot. The results are expressed as relative expression against negative control expression. Data represent the mean ± SD from three independent experiments. ⁎p b 0.05; ⁎⁎p b 0.01 vs 0 ng/ml group. b. HSC-T6 cells were stimulated with TGF-β1 (10 ng/ml) for 0,12,24 and 48 h. The mRNA level of PTP1B, Col1a1 and α-SMA were examined by Real-time PCR; the protein level of PTP1B, Col1a1 and α-SMA were assessed by Western blot. The results are expressed as relative expression against negative control expression. Data represent the mean ± SD from three independent experiments. ⁎p b 0.05; ⁎⁎p b 0.01 vs 0 h group.

Article Snippet: Rabbit polyclonal anti-aSMA (Proteintech, USA) was diluted at 1:500, Rabbit polyclonal anti -PTP1B and Col1α1 (Boster, China) were diluted 1:200; mouse monoclonal anti-β-actin was diluted 1:200.

Techniques: Expressing, Activation Assay, Real-time Polymerase Chain Reaction, Western Blot, Negative Control

Journal: Toxicology and applied pharmacology

Article Title: PTP1B confers liver fibrosis by regulating the activation of hepatic stellate cells.

doi: 10.1016/j.taap.2015.12.021

Figure Lengend Snippet: Fig. 4. Effect of PTP1B on cell proliferation, cell cycle and apoptosis in TGF- β1-treated HSC-T6 cells. a. Reduced expression of PTP1B in HSC-T6 cells by PTP1B-siRNA. The results are expressed as relative expression against control expression. Data represent the mean ± SD from three independent experiments. ⁎⁎p b 0.01 vs Scrambled-siRNA group. b. Inhibition of PTP1B significantly decreased cell proliferation of TGF-β1-treated HSC-T6 cells. Proliferation of HSC-T6 cells was tested by MTT assay. Data represent the mean ± SD from three independent experiments.⁎p b 0.05 vs TGF-β1 + Scrambled-siRNA group. c. Effect of PTP1B on cell cycle in TGF-β1-treated HSC-T6 cells. The results are expressed as relative expression against control expression without treatment. Representative images of three independent experiments are shown. ##p b 0.01vs TGF-β1 + Scrambled-siRNA group. d. Effect of PTP1B on apoptosis in TGF- β1-treated HSC-T6 cells. The results are expressed as relative expression against control expression without treatment. One representative experiment of the three independent experiments is demonstrated.

Article Snippet: Rabbit polyclonal anti-aSMA (Proteintech, USA) was diluted at 1:500, Rabbit polyclonal anti -PTP1B and Col1α1 (Boster, China) were diluted 1:200; mouse monoclonal anti-β-actin was diluted 1:200.

Techniques: Expressing, Control, Inhibition, MTT Assay

Journal: Toxicology and applied pharmacology

Article Title: PTP1B confers liver fibrosis by regulating the activation of hepatic stellate cells.

doi: 10.1016/j.taap.2015.12.021

Figure Lengend Snippet: Fig. 5. Blockade of PTP1B decreased TGF-β1-induced expressions of α-SMA and Col1 α1 in HSC-T6 cells. After PTP1B-siRNA or Scrambled-siRNA transfection, then exposure HSC-T6 cells to TGF-β1 (10 ng/ml) for 24 h. (a) Real-time PCR were performed to examine the mRNA level of PTP1B, Col1a1 and α-SMA. (b) Western blot was performed to assess the protein level of PTP1B, Col1a1 and α-SMA. The results are expressed as relative expression against negative control expression. Data represent the mean ± SD from three independent experiments. ⁎⁎p b 0.01 vs Control; ##p b 0.01 vs Scrambled-siRNA.

Article Snippet: Rabbit polyclonal anti-aSMA (Proteintech, USA) was diluted at 1:500, Rabbit polyclonal anti -PTP1B and Col1α1 (Boster, China) were diluted 1:200; mouse monoclonal anti-β-actin was diluted 1:200.

Techniques: Transfection, Real-time Polymerase Chain Reaction, Western Blot, Expressing, Negative Control, Control

Journal: Toxicology and applied pharmacology

Article Title: PTP1B confers liver fibrosis by regulating the activation of hepatic stellate cells.

doi: 10.1016/j.taap.2015.12.021

Figure Lengend Snippet: Fig. 6. Decreased expression of PTP1B in inactivated HSC-T6 cells. HSC-T6 cells were activated by TGF-β1 (10 ng/ml, 24 h), then they were treated with MDI for 48 h to be inactivated. The mRNA level of PTP1B, Col1a1 and α-SMA were examined by Real-time PCR (a); the protein level of PTP1B, Col1a1 and α-SMA were assessed by Western blot (b). The results are expressed as relative expression against negative control expression. Data represent the mean ± SD from three independent experiments. ⁎p b 0.05, ⁎⁎p b 0.01 vs control; #p b 0.05, ##p b 0.01 vs TGF-β1.

Article Snippet: Rabbit polyclonal anti-aSMA (Proteintech, USA) was diluted at 1:500, Rabbit polyclonal anti -PTP1B and Col1α1 (Boster, China) were diluted 1:200; mouse monoclonal anti-β-actin was diluted 1:200.

Techniques: Expressing, Real-time Polymerase Chain Reaction, Western Blot, Negative Control, Control

Journal: Toxicology and applied pharmacology

Article Title: PTP1B confers liver fibrosis by regulating the activation of hepatic stellate cells.

doi: 10.1016/j.taap.2015.12.021

Figure Lengend Snippet: Fig. 7. Over-expression of PTP1B reversed the inactivation of HSC-T6 cells induced by MDI. HSC-T6 cells were activated by TGF-β1 (10 ng/ml, 24 h), then they were transfected with pCDNA3.1-PTP1B or pCDNA3.1-control plasmid. After that, cells were treated with MDI for 48 h to be inactivated. The mRNA level of PTP1B, Col1a1 and α-SMA were examined by Real-time PCR (a); the protein level of PTP1B, Col1a1 and α-SMA were assessed by Western blot (b). The results are expressed as relative expression against negative control expression. Data represent the mean ± SD from three independent experiments. ⁎p b 0.05, ⁎⁎p b 0.01 vs TGF-β1; #p b 0.05, ##p b 0.01 vs pCDNA3.1-control.

Article Snippet: Rabbit polyclonal anti-aSMA (Proteintech, USA) was diluted at 1:500, Rabbit polyclonal anti -PTP1B and Col1α1 (Boster, China) were diluted 1:200; mouse monoclonal anti-β-actin was diluted 1:200.

Techniques: Over Expression, Transfection, Control, Plasmid Preparation, Real-time Polymerase Chain Reaction, Western Blot, Expressing, Negative Control

Journal: Frontiers in Immunology

Article Title: PTPN1 is a prognostic biomarker related to cancer immunity and drug sensitivity: from pan-cancer analysis to validation in breast cancer

doi: 10.3389/fimmu.2023.1232047

Figure Lengend Snippet: Expression landscape of PTPN1 in pan-cancer. (A) Distribution of PTPN1 expression in various cancer types from TCGA database. (B) PTPN1 expression level in various cancer cell lines from the CCLE database. (C) Representative images of immunofluorescence staining of PTPN1 protein in the nucleus, endoplasmic reticulum (ER), and microtubules in A-431 and U-251 cell lines from the HPA database. * P < 0.05, ** P < 0.01, *** P < 0.001 by Student’s t-test.

Article Snippet: The immunofluorescence staining images of PTPN1 in two human cancer cell lines (A-431 and U-251MG) were downloaded from the Human Protein Atlas (HPA; www.proteinatlas.org ) online platform.

Techniques: Expressing, Immunofluorescence, Staining

Journal: Frontiers in Immunology

Article Title: PTPN1 is a prognostic biomarker related to cancer immunity and drug sensitivity: from pan-cancer analysis to validation in breast cancer

doi: 10.3389/fimmu.2023.1232047

Figure Lengend Snippet: Relationship between PTPN1 expression and OS in pan-cancer. (A) The relationship between PTPN1 expression and OS in the cancer types indicated was analyzed using univariate Cox regression analyses. (B) The impact of PTPN1 on OS in the cancer types indicated was assessed using Kaplan-Meier survival curves. (C) Time-dependent ROC curve analysis was used to assess the performance of PTPN1 in predicting 1-, 3-, and 5-year OS for the cancer types indicated.

Article Snippet: The immunofluorescence staining images of PTPN1 in two human cancer cell lines (A-431 and U-251MG) were downloaded from the Human Protein Atlas (HPA; www.proteinatlas.org ) online platform.

Techniques: Expressing

Journal: Frontiers in Immunology

Article Title: PTPN1 is a prognostic biomarker related to cancer immunity and drug sensitivity: from pan-cancer analysis to validation in breast cancer

doi: 10.3389/fimmu.2023.1232047

Figure Lengend Snippet: Relationship between PTPN1 expression and DSS in pan-cancer. (A) The relationship between PTPN1 expression and DSS in the cancer types indicated was analyzed using univariate Cox regression analysis. (B) Kaplan-Meier survival curves showing the impact of PTPN1 on DSS in the cancer types indicated. (C) The performance of PTPN1 in predicting the 1-, 3-, and 5-year DSS for the cancer types indicated was evaluated using time-dependent ROC curve analysis.

Article Snippet: The immunofluorescence staining images of PTPN1 in two human cancer cell lines (A-431 and U-251MG) were downloaded from the Human Protein Atlas (HPA; www.proteinatlas.org ) online platform.

Techniques: Expressing

Journal: Frontiers in Immunology

Article Title: PTPN1 is a prognostic biomarker related to cancer immunity and drug sensitivity: from pan-cancer analysis to validation in breast cancer

doi: 10.3389/fimmu.2023.1232047

Figure Lengend Snippet: Relationship between PTPN1 expression and immune subtypes in cancers.

Article Snippet: The immunofluorescence staining images of PTPN1 in two human cancer cell lines (A-431 and U-251MG) were downloaded from the Human Protein Atlas (HPA; www.proteinatlas.org ) online platform.

Techniques: Expressing

Journal: Frontiers in Immunology

Article Title: PTPN1 is a prognostic biomarker related to cancer immunity and drug sensitivity: from pan-cancer analysis to validation in breast cancer

doi: 10.3389/fimmu.2023.1232047

Figure Lengend Snippet: Correlation between PTPN1 level and immune infiltrates in various cancers. (A, B) Radar charts show the relationship between PTPN1 expression and stromal score (A) and immune score (B) in pan-cancer. (C) The heat map shows the correlation between the expression level of PTPN1 and the presence of immune infiltrates in pan-cancer.

Article Snippet: The immunofluorescence staining images of PTPN1 in two human cancer cell lines (A-431 and U-251MG) were downloaded from the Human Protein Atlas (HPA; www.proteinatlas.org ) online platform.

Techniques: Expressing

Journal: Frontiers in Immunology

Article Title: PTPN1 is a prognostic biomarker related to cancer immunity and drug sensitivity: from pan-cancer analysis to validation in breast cancer

doi: 10.3389/fimmu.2023.1232047

Figure Lengend Snippet: Correlation between PTPN1 level and expression of immune checkpoint-related genes in various cancers. (A) The heat map shows the correlation between the expression levels of PTPN1 and immune checkpoint genes in pan-cancer. (B, C) Radar charts show the relationship between PTPN1 expression and TMB (B) and MSI (C) in pan-cancer. (D) Kaplan-Meier curve showing the impact of PTPN1 on OS of patients from the IMvigor210, GSE78220, and GSE91061 cohorts. * P < 0.05; ** P < 0.01; *** P < 0.001.

Article Snippet: The immunofluorescence staining images of PTPN1 in two human cancer cell lines (A-431 and U-251MG) were downloaded from the Human Protein Atlas (HPA; www.proteinatlas.org ) online platform.

Techniques: Expressing

Journal: Frontiers in Immunology

Article Title: PTPN1 is a prognostic biomarker related to cancer immunity and drug sensitivity: from pan-cancer analysis to validation in breast cancer

doi: 10.3389/fimmu.2023.1232047

Figure Lengend Snippet: PTPN1 expression correlated with immune infiltration in human breast cancer tissues. (A) Representative PTPN1 staining in human breast cancer tissues and adjacent normal tissues (scale bars: top, 200 μm; bottom, 50 μm). (B) The immunoreactivity scores of PTPN1 in normal breast tissues and breast cancer tissues were compared using the Mann-Whitney U test (** P < 0.01). (C) Representative IHC staining images for PTPN1, CD68, and CD163 expression in three serial sections of the same tumor from two primary human breast cancer specimens (scale bars: top, 200 μm; bottom, 50 μm). (D) Representative IHC staining images for PTPN1, CD8, and PD-L1 expression in three serial sections of the same tumor from two primary human breast cancer specimens (scale bars: top, 200 μm; bottom, 50 μm.). (E) Quantitative determination of CD68 + CD163 + cells in the PTPN1 high- and low-expression groups. (F) The number of CD8 + T cells in breast cancer tissues with high or low PTPN1 expression. (G) The correlation between the expression levels of PTPN1 and PD-L1 proteins was evaluated using Pearson’s correlation. Error bars, SEM. * P < 0.05, *** P < 0.001 using Student’s t-test.

Article Snippet: The immunofluorescence staining images of PTPN1 in two human cancer cell lines (A-431 and U-251MG) were downloaded from the Human Protein Atlas (HPA; www.proteinatlas.org ) online platform.

Techniques: Expressing, Staining, MANN-WHITNEY, Immunohistochemistry

Journal: Frontiers in Immunology

Article Title: PTPN1 is a prognostic biomarker related to cancer immunity and drug sensitivity: from pan-cancer analysis to validation in breast cancer

doi: 10.3389/fimmu.2023.1232047

Figure Lengend Snippet: PTPN1 expression correlated with tumor growth and tumor immune infiltration in vivo . (A) PTPN1 knockdown in 4T1 cells was confirmed using immunoblotting. β-actin was used as the internal loading control. (B) 4T1-shPTPN1 and 4T1-shCtrl cells were subcutaneously injected into BALB/c mice, and tumor growth was monitored for 28 days. (C) Representative images of tumors derived from mice. (D) Representative images of immunofluorescence staining of CD163 (green) and F4/80 (red) in BALB/c mouse tumor tissues from 4T1-shPTPN1 and 4T1-shCtrl cells (scale bars: 100 μm). (E) Quantitative determination of F4/80 + CD163 + cells in tumor tissues of 4T1-shPTPN1 versus 4T1-shCtrl cells. (F) Representative immunofluorescence images of CD8 (green) and PD-L1 (red) expression in BALB/c mouse tumor tissues from 4T1-shPTPN1 and 4T1-shCtrl cells (scale bars: 200 μm). (G) The number of CD8 + T cells in tumor tissues of 4T1-shPTPN1 versus 4T1-shCtrl cells. (H) Quantitative determination of PD-L1 protein expression in tumor tissues of 4T1-shPTPN1 versus 4T1-shCtrl cells. Error bars, SEM. * P < 0.05, ** P < 0.01, *** P < 0.001 using Student’s t-test.

Article Snippet: The immunofluorescence staining images of PTPN1 in two human cancer cell lines (A-431 and U-251MG) were downloaded from the Human Protein Atlas (HPA; www.proteinatlas.org ) online platform.

Techniques: Expressing, In Vivo, Knockdown, Western Blot, Control, Injection, Derivative Assay, Immunofluorescence, Staining

Journal: Frontiers in Immunology

Article Title: PTPN1 is a prognostic biomarker related to cancer immunity and drug sensitivity: from pan-cancer analysis to validation in breast cancer

doi: 10.3389/fimmu.2023.1232047

Figure Lengend Snippet: Correlation between PTPN1 level and drug sensitivity in cancer cells. (A, B) The correlation between PTPN1 expression and chemotherapeutic drug sensitivity in cancer cells was investigated using the data from the CellMiner (A) and GDSC databases (B) . (C) The mRNA level of PTPN1 in MDA-MB-231 and MCF-7 cells with PTPN1 knockdown was examined using qRT-PCR. (D) The protein level of PTPN1 in MDA-MB-231 and MCF-7 cells with PTPN1 knockdown was examined using immunoblotting. β-actin was used as the internal loading control. (E) The cell inhibition ratios of MDA-MB-231 and MCF-7 cells with PTPN1 knockdown were evaluated using the CCK-8 viability assay. Cells were treated with various concentrations of paclitaxel (0, 5, 10, 15, 20, 25, and 30 µM) for 72 h. Data are shown as the means of three independent experiments. Error bars indicate SEM. * P < 0.05, ** P < 0.01, *** P < 0.001 using one-way ANOVA with post hoc intergroup comparisons.

Article Snippet: The immunofluorescence staining images of PTPN1 in two human cancer cell lines (A-431 and U-251MG) were downloaded from the Human Protein Atlas (HPA; www.proteinatlas.org ) online platform.

Techniques: Expressing, Knockdown, Quantitative RT-PCR, Western Blot, Control, Inhibition, CCK-8 Assay, Viability Assay